December 28, 2017


ME244: Experimental methods in Microfluidics (Jan 2018)

    Announcements:December 30,2017: Organizational meeting and first lecture will be held in ME Lecture Hall from 2 pm on January 2nd (Tuesday).

      January 3,2018: Report to lecture hall for Jan 4rth. Any change in class location will be intimated at class time.

      January 9,2018: Classroom is now shifted to MMCR. This is the lecture room on the 1st floor of the building opposite to the main mechanical engineering building.

      January 25,2018: Homework 1 is now available.Homework 1 Image Set HomeWork 1

      February 8,2018: Homework 2 is now available. Homework 2

      February 13,2018:Feb 13 is a holiday (Shivratri). There will be no classes.

      February 13,2018:Image 1Image 2Homework 3 PDF

      March 06,2018: Homework 1 will be returned and discussed. Please prepare (pre) project presentations (short 5-10 mins). Only feedback will be provided.

      March 06,2018: Homework 3 extension – Can be returned on March 8 at 2 pm

      March 20,2018: Homework 4 is now available.HW4

      Homework 4 deadline extended to April 3rd (2 pm).

      Project presentations will be held (tentatively) on April 15th. Details to be discussed in class.

      April 2,2018:Homework 3 solution

      April 03,2018:Homework 3 discussion. DIC Class demo. Announcement regarding projects and exam.

      April 05,2018:DIC Image HW5

      Instructor: Aloke Kumar
      Office: MECH 401
      Phone: +91(80)22932958
      Email: alokekumar AT

      Class Meeting: Tuesdays and Thursdays 2-3.30 pm (Venue: ME Lecture Hall)

      About the course:
      Introduction to experimental methods used in microfluidic systems. Fundamentals of flows at the microscale; emphasis on visualization and quantification of fluid flow at the micron-scale. Brownian motion and its quantification. Particle image velocimetry (PIV), micro-particle image velocimetry (µ-PIV) and three-component flow measurement in three dimensions. Measuring displacement at the micron scale; digital image correlation (DIC). Thermometry at the micron-scale; laser induced fluorescence (LIF). Applications to microfluidic, biomicrofluidic and biomechanics.

      Details of course topics:
      • Introduction: Introduction to microfluidics and the challenges and novelty that miniaturization brings. General concepts of microscale fluid flows will also be discussed.
      • General concepts on flow visualization: Using dyes, and other visualization techniques to observe flows and flow structures. Introduction to the basics of different optical techniques, such as fluorescence imaging.
      • Particle-tracking and Particle-image velocimetry: Fundamentals of particle tracking and particle-image velocimetry (PIV). Details of correlation tracking.
      • Microscale flow visualization – Issues and difficulties with microscale flow visualization. How to adopt PIV for micro-scale flow visualization?
      • Three-dimensional flow visualization – How to visualize and quantify flow in three-dimensions. Details of two-camera PIV imaging. Aberration based three-dimensional particle tracking velocimetry.
      • Strain measurement at the micron-scale – Introduction to digital image correlation (DIC) and related optical methods for strain measurement.
      • Thermometry: Measurement of temperature at the micron scale using techniques such as LIF fluorescence will be discussed.

      Background in fluid mechanics and transport phenomena is encouraged. Knowledge of statistical techniques will be beneficial, but not required.

      References: (principal: instructor’s notes)
      Raffel, M., Willert, C., Wereley, S.T., Kompenhans, J, Particle Image Velocimetry, Springer, 2007
      Nguyen, Nam-Trung, Wereley, S.T., Fundamentals and Applications of Microfluidics, Artech House, 2006
      Li, Dongqing (Ed), Encyclopedia of Microfluidics and Nanofluidics, Springer, 2008

      Academic Honesty: As students of IISc, we expect you to adhere to the highest standards of academic honesty and integrity.

      Freaky Dot Patterns (Youtube Link)
      Leaping Shampoo (Youtube Link)
      Rapid Electrokinetic Patterning aka Brahma Tweezers (Youtube Link)
      Digital Image Correlation (Youtube Link)
      Hydraulic Jump (Youtube video)
      Download image
      Deconvolution Microscopy(Youtube video)
      Bead in an Optical Trap